Publications

Druggable fusion gene landscape in solid tumors

J Clin Oncol 38: 2020 (suppl; abstr e13517) DOI:

https://doi.org/10.1200/JCO.2020.38.15_suppl.e13517

Williams GH, Thatcher RP, Nevado B, Haddow TE, Hardisty KM, Loddo M (2020)

Consistency of tumor and immune cell programmed cell death ligand-1 expression within and between tumor blocks using the VENTANA SP263 assay

Diagnostic Pathology, (2018) 13:47 DOI:

https://doi.org/10.1186/s13000-018-0725-9

Paul Scorer, Marietta Scott, Nicola Lawson et al (2018)

DNA replication licensing factor MCM2, geminin, and Ki67 define proliferative state and are linked with survival in oral squamous cell carcinoma

Eur J Oral Sci. 2018 Jun;126(3):186-196. doi:

https://doi.org/10.1111/eos.12420

Al-Hazmi N, Alhazzazi T, Williams G, Stoeber K, Al-Dabbagh R

Subcellular proteomics reveals a role for nucleo-cytoplasmic trafficking at the DNA replication origin activation checkpoint

J Proteome Res. 2013 Mar 1;12(3):1436-53

Mulvey CM, Tudzarova S, Crawford M, Williams GH, Stoeber K, Godovac-Zimmermann J

Pregnancy-associated plasma protein A regulates mitosis and is epigenetically silenced in breast cancer

J Pathol. 2014 Aug;233(4):344-56.

Loddo M, Andryszkiewicz J, Rodriguez-Acebes S, Stoeber K, Jones A, Dafou D, Apostolidou S, Wollenschlaeger A, Widschwendter M, Sainsbury R, Tudzarova S, Williams GH.

Assessment of PD-L1 mRNA and protein expression in non-small cell lung cancer, head and neck squamous cell carcinoma and urothelial carcinoma tissue specimens using RNAScope and immunohistochemistry.

PLoS ONE 14(4): e0215393

David Jonathan Duncan ,Marietta Scott,Paul Scorer et al ()

Cdc7 is a potent anti-cancer target in pancreatic cancer due to abrogation of the DNA origin activation checkpoint

Oncotarget. 2016 Feb 23. doi:

https://doi.org/10.18632/oncotarget.7611

Huggett MT, Tudzarova S, Proctor I, Loddo M, Keane MG, Stoeber K, Williams GH, Pereira SP.

Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment

Br J Haematol. 2016 Apr 8. doi:

https://doi.org/10.1111/bjh.14055

Sellar RS, Fraser L, Khwaja A, Gale RE, Marafioti T, Akarca A, Hubank M, Brooks T, Stoeber K,Williams G, Linch DC

Exploring the interaction between siRNA and the SMoC biomolecule transporters: implications for small molecule-mediated delivery of siRNA

Chem Biol Drug Des. 2012 Jan;79(1):9-21.

Gooding M, Tudzarova S, Worthington RJ, Kingsbury SR, Rebstock AS, Dube H, Simone MI, Visintin C, Lagos D, Quesada JM, Laman H, Boshoff C, Williams GH, Stoeber K, Selwood DL.

Integration of next-generation sequencing in clinical diagnostic molecular pathology laboratories for analysis of solid tumours; an expert opinion on behalf of IQN Path ASBL

Virchows Arch (2016). doi:

https://doi.org/10.1007/s00428-016-2025-7

Zandra C Deans, Jose Luis Costa, Ian Cree, Els Dequeker, Anders Edsjö, Shirley Henderson, Michael Hummel, Marjolijn JL Ligtenberg, Marco Loddo, Jose Carlos Machado, Antonio Marchetti, Katherine Marquis, Joanne Mason, Nicola Normanno, Etienne Rouleau, Ed Schuuring, Keeda-Marie Snelson, Erik Thunnissen, Bastiaan Tops, Gareth Williams, Han van Krieken, Jacqueline A Hall, On behalf of IQN Path ASBL

The cell cycle and cancer

J Pathol. 2012 Jan;226(2):352-64.

Williams GH, Stoeber K.

Bladder cancer diagnosis and identification of clinically significant disease by combined urinary detection of Mcm5 and nuclear matrix protein 22

PLoS One. 2012;7(7):e40305

Kelly JD, Dudderidge TJ, Wollenschlaeger A, Okoturo O, Burling K, Tulloch F, Halsall I, Prevost T, Prevost AT, Vasconcelos JC, Robson W, Leung HY, Vasdev N, Pickard RS, Williams GH, Stoeber K.

A comparative study of PD-L1 diagnostic assays in squamous cell carcinoma of the head and neck (SCCHN)

Annals of Oncology; 27 (suppl_6): 955PD DOI:

https://doi.org/10.1093/annonc/mdw376.07

J. Ratcliffe, A. Sharpe, M. Rebelatto et al ()

A novel p53-Cdc7 link induced by genotoxic stress

Cell Cycle, DOI:

https://doi.org/10.1080/15384101.2017.1304746

Hisao Masai

Utilisation of semiconductor sequencing for the detection of predictive biomarkers in glioblastoma

Published: March 25, 2022 DOI:

https://doi.org/10.1371/journal.pone.0245817

Gareth H Williams, Alexander Llewelyn, Robert Thatcher, Keeda-Marie Hardisty, Marco Loddo

Immunophenotypic analysis of cell cycle status in acute myeloid leukaemia: relationship to cytogenetics, genotype and clinical outcome

Br J Haematol. 2018 May;181(4):486-494 [Epub 2018 Apr 20.]

https://doi.org/10.1111/bjh.15211

Sellar RS, Gale RE, Khwaja A, Garbowski M, Loddo M, Stoeber K, Williams GH, Linch DC

The actionable genomic mutational landscape in solid tumours

J Clin Oncol 38: 2020 (suppl; abstr e13642) DOI:

https://doi.org/10.1200/JCO.2020.38.15_suppl.e13642

Loddo M, Hardisty KM, Thatcher RP, Haddow TE, Williams GH (2020)

Agreement between Programmed Cell Death Ligand-1 Diagnostic Assays across Multiple Protein Expression Cut-Offs in Non-Small Cell Lung Cancer

Clinical Cancer Research, January 2017; 16-2375 DOI:

https://doi.org/10.1158/1078-0432.ccr-16-2375

Marianne J Ratcliffe, Alan Sharpe, Anita Midha et al (2017)

p53 controls CDC7 levels to reinforce G1 cell cycle arrest upon genotoxic stress

Cell Cycle. 2016 Sep 9:0 DOI:

https://doi.org/10.1080/15384101.2016.1231281

Tudzarova S, Mulholland P, Dey A, Stoeber K, Okorokov AL, Williams GH

Clinical, Radiometabolic and Immunologic Effects of Olaparib in Locally Advanced Triple Negative Breast Cancer: The OLTRE Window of Opportunity Trial

Front Oncol. 2021; 11: 686776. Published online 2021 Jun 28. doi:

https://doi.org/10.3389/fonc.2021.686776

Alfredo Molteni, Alice Bruson, Carla Strina, Carlo Azzini, Daniele Generali, Davide Cerra, Elena Marasco, Elisa Zago, Fabiola Giudici, Francesco Schettini, Gareth H Williams, Giovanni Scambia, Giuseppina Barbieri, Giuseppina Ferrero, Ida Paris, Luciano Xumerle, Manuela Milani, Marco Loddo, Marco Ungari, Maria Chiara Lazzari, Maria Rosa Cappelletti, Marianna Sirico, Nicoletta Ziglioli, Ottavia Bernocchi, Sergio Aguggini, Silvia Paola Corona, Valeria Cervoni

Do Cell-Cycle Phase-Specific Markers Predict Disease Grade, Stage, and Outcome in Cervical Carcinoma?

Int J Gynecol Cancer. 2015 Jul;25(6):1066-72

Kuku S, Proctor I, Loddo M, Kadalayil L, KhoshZaban M, Ledermann J, McCormack M.

Molecular architecture of the DNA replication origin activation checkpoint

EMBO J. 2010 Oct 6;29(19):3381-94.

Tudzarova S, Trotter MW, Wollenschlaeger A, Mulvey C, Godovac-Zimmermann J, Williams GH, Stoeber K.

Inter-observer Reliability of Programmed Cell Death Ligand-1 Scoring Using the VENTANA PD-L1 (SP263) Assay in Non-Small Cell Lung Cancer

J Thorac Oncol. 2019 Nov 25. pii: S1556-0864(19)33664-0. doi:

https://doi.org/10.1016/j.jtho.2019.11.010

Williams GH, Nicholson AG, Snead DRJ, Thunnissen E, Lantuejoul S, Cane P, Kerr KM, Loddo M, Scott MLJ, Scorer PW, Barker C. (2019)

Immunofocus-PD-L1 high throughput quantitative next generation sequencing assay

J Clin Oncol 38: 2020 (suppl; abstr e13521) DOI:

https://doi.org/10.1200/JCO.2020.38.15_suppl.e13521

Williams GH, Thatcher RP, Haddow TE, Hardisty KM, Loddo M (2020)

Relevance of deep learning to facilitate the diagnosis of HER2 status in breast cancer.

Nature – Scientific Reports, April 2017, 7, 45938 DOI:

https://doi.org/10.1038/srep45938

Michel E. Vandenberghe, Marietta L. J. Scott, Paul W. Scorer et al (2017)

Targeting DNA replication before it starts: Cdc7 as a therapeutic target in p53-mutant breast cancers

Am J Pathol. 2010 Oct;177(4):2034-45.

Rodriguez-Acebes S, Proctor I, Loddo M, Wollenschlaeger A, Rashid M, Falzon M, Prevost AT, Sainsbury R, Stoeber K, Williams GH.

Two ubiquitin ligases, APC/C-Cdh1 and SKP1-CUL1-F (SCF)-beta-TrCP, sequentially regulate glycolysis during the cell cycle

Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5278-83

Tudzarova S, Colombo SL, Stoeber K, Carcamo S, Williams GH, Moncada S.

Mapping the binding sites of antibodies utilized in programmed cell death ligand-1 predictive immunohistochemical assays for use with immuno-oncology therapies

Mod Pathol. 2019 Sep 26. doi [Epub ahead of print] Erratum in: Mod Pathol. 2019 Oct:

https://doi.org/10.1038/s41379-019-0372-z

Lawson NL, Dix CI, Scorer PW, Stubbs CJ, Wong E, Hutchinson L, McCall EJ, Schimpl M, DeVries E, Walker J, Williams GH, Hunt J, Barker C. (2019)

SARS-CoV-2 testing and sequencing for international arrivals reveals significant cross border transmission of high risk variants into the United Kingdom

EClinical Medicine by the Lancet Published:July 14, 2021 DOI:

https://doi.org/10.1016/j.eclinm.2021.101021

Gareth H Williams, Alexander Llewelyn, Ruben Brandao, Kaiya Chowdhary,Keeda-Marie Hardisty, Marco Loddo (2021)

Molecular structure of human geminin

Nat Struct Mol Biol. 2004 Oct;11(10):1021-2.

Okorokov AL, Orlova EV, Kingsbury SR, Bagneris C, Gohlke U, Williams GH, Stoeber K.

Hexameric ring structure of human MCM10 DNA replication factor

EMBO Rep. 2007 Oct;8(10):925-30.

Okorokov AL, Waugh A, Hodgkinson J, Murthy A, Hong HK, Leo E, Sherman MB, Stoeber K, Orlova EV, Williams GH.

DNA replication licensing in peripheral B-cell lymphoma

J Pathol. 2005 Feb;205(3):318-28.

Obermann EC, Eward KL, Dogan A, Paul EA, Loddo M, Munson P, Williams GH, Stoeber K.

DNA replication licensing in somatic and germ cells

J Cell Sci. 2004 Nov 15;117(Pt 24):5875-86.

Eward KL, Obermann EC, Shreeram S, Loddo M, Fanshawe T, Williams C, Jung HI, Prevost AT, Blow JJ, Stoeber K, Williams GH.

Cell cycle markers in clinical oncology

Curr Opin Cell Biol. 2007 Dec;19(6):672-9.

Williams GH, Stoeber K.

Cdc6 protein causes premature entry into S phase in a mammalian cell-free system

EMBO J. 1998 Dec 15;17(24):7219-29.

Stoeber K, Mills AD, Kubota Y, Krude T, Romanowski P, Marheineke K, Laskey RA, Williams GH.

Identification of an arginine-rich motif in human papillomavirus type 1 E1;E4 protein necessary for E4-mediated inhibition of cellular DNA synthesis in vitro and in cells

J Virol 82:9056-9064

Roberts S, Kingsbury SR, Stoeber K, Knight GL Gallimore PH & Williams GH

Standard Reference Material: Cell Line Development and Use of Reference Cell Lines as Standards for External Quality Assurance of Her2 IHC and ISH Testing. Antigen Retrieval Immunohistochemistry Based Research and Diagnostics (Book)

First Edition; 2010; Chapter 6; pp.101-122 DOI:

https://doi.org/10.1002/9780470875612.ch6

Shan-Rong Shi and Clive R. Taylor (2010)

Use of the Monoclonal Antibody DAKO-ERβ (8D5-1) to Measure Oestrogen Receptor Beta in Breast Cancer Cells

Cytometry, 2001, Vol. 45: p65-72 DOI:

https://doi.org/10.1002/1097-0320(20010901)45:1%3C65::aid-cyto1145%3E3.0.co;2-8

F. Girdler, D.A. Browell W.J. Cunliffe et al (2001)

Cdc7 kinase is a predictor of survival and a novel therapeutic target in epithelial ovarian carcinoma

Clin Cancer Res. 2009 Apr 1;15(7):2417-25.

Kulkarni AA, Kingsbury SR, Tudzarova S, Hong HK, Loddo M, Rashid M, Rodriguez-Acebes S, Prevost AT, Ledermann JA, Stoeber K, Williams GH.

Small-molecule mimics of an alpha-helix for efficient transport of proteins into cells

Nat Methods. 2007 Feb;4(2):153-9.

Okuyama M, Laman H, Kingsbury SR, Visintin C, Leo E, Eward KL, Stoeber K, Boshoff C, Williams GH, Selwood DL.

Aberrant expression of minichromosome maintenance proteins 2 and 5, and Ki-67 in dysplastic squamous oesophageal epithelium and Barrett’s mucosa

Gut. 2002 Mar;50(3):373-7.

Going JJ, Keith WN, Neilson L, Stoeber K, Stuart RC, Williams GH.

Phospho-STAT5 and phospho-Akt expression in chronic myeloproliferative neoplasms

British Journal of Haematology, 2009, Vol. 147: p495-506 DOI:

https://doi.org/10.1111/j.1365-2141.2009.07870.x

L. F. Grimwade, L. Happerfield, C. Tristram Lisa Happerfield et al (2009)

Diagnosis of pancreaticobiliary malignancy by detection of minichromosome maintenance protein 5 in bile aspirates

Br J Cancer. 2008 May 6;98(9):1548-54.

Ayaru L, Stoeber K, Webster GJ, Hatfield AR, Wollenschlaeger A, Okoturo O, Rashid M, Williams G, Pereira SP.

DNA replication licensing and human cell proliferation

J Cell Sci. 2001 Jun;114(Pt 11):2027-41.

Stoeber K, Tlsty TD, Happerfield L, Thomas GA, Romanov S, Bobrow L, Williams ED, Williams GH.

Immunoassay for urothelial cancers that detects DNA replication protein Mcm5 in urine

Lancet. 1999 Oct 30;354(9189):1524-5.

Stoeber K, Halsall I, Freeman A, Swinn R, Doble A, Morris L, Coleman N, Bullock N, Laskey RA, Hales CN, Williams GH.

Novel markers of cell kinetics to evaluate progression from cirrhosis to hepatocellular carcinoma

Liver Int. 2006 May;26(4):424-32.

Quaglia A, McStay M, Stoeber K, Loddo M, Caplin M, Fanshawe T, Williams G, Dhillon A.

DNA replication licensing factors and aurora kinases are linked to aneuploidy and clinical outcome in epithelial ovarian carcinoma.

Clin Cancer Res. 2007 Oct 15;13(20):6153-61.

Kulkarni AA, Loddo M, Leo E, Rashid M, Eward KL, Fanshawe TR, Butcher J, Frost A, Ledermann JA, Williams GH, Stoeber K.

Publications

Druggable fusion gene landscape in solid tumors

Consistency of tumor and immune cell programmed cell death ligand-1 expression within and between tumor blocks using the VENTANA SP263 assay

DNA replication licensing factor MCM2, geminin, and Ki67 define proliferative state and are linked with survival in oral squamous cell carcinoma

Subcellular proteomics reveals a role for nucleo-cytoplasmic trafficking at the DNA replication origin activation checkpoint

Pregnancy-associated plasma protein A regulates mitosis and is epigenetically silenced in breast cancer

Assessment of PD-L1 mRNA and protein expression in non-small cell lung cancer, head and neck squamous cell carcinoma and urothelial carcinoma tissue specimens using RNAScope and immunohistochemistry.

Cdc7 is a potent anti-cancer target in pancreatic cancer due to abrogation of the DNA origin activation checkpoint

Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment

Exploring the interaction between siRNA and the SMoC biomolecule transporters: implications for small molecule-mediated delivery of siRNA

Integration of next-generation sequencing in clinical diagnostic molecular pathology laboratories for analysis of solid tumours; an expert opinion on behalf of IQN Path ASBL

The cell cycle and cancer

Bladder cancer diagnosis and identification of clinically significant disease by combined urinary detection of Mcm5 and nuclear matrix protein 22

A comparative study of PD-L1 diagnostic assays in squamous cell carcinoma of the head and neck (SCCHN)

A novel p53-Cdc7 link induced by genotoxic stress

Utilisation of semiconductor sequencing for the detection of predictive biomarkers in glioblastoma

Immunophenotypic analysis of cell cycle status in acute myeloid leukaemia: relationship to cytogenetics, genotype and clinical outcome

The actionable genomic mutational landscape in solid tumours

Agreement between Programmed Cell Death Ligand-1 Diagnostic Assays across Multiple Protein Expression Cut-Offs in Non-Small Cell Lung Cancer

p53 controls CDC7 levels to reinforce G1 cell cycle arrest upon genotoxic stress

Clinical, Radiometabolic and Immunologic Effects of Olaparib in Locally Advanced Triple Negative Breast Cancer: The OLTRE Window of Opportunity Trial

Do Cell-Cycle Phase-Specific Markers Predict Disease Grade, Stage, and Outcome in Cervical Carcinoma?

Molecular architecture of the DNA replication origin activation checkpoint

Inter-observer Reliability of Programmed Cell Death Ligand-1 Scoring Using the VENTANA PD-L1 (SP263) Assay in Non-Small Cell Lung Cancer

Immunofocus-PD-L1 high throughput quantitative next generation sequencing assay

Relevance of deep learning to facilitate the diagnosis of HER2 status in breast cancer.

Targeting DNA replication before it starts: Cdc7 as a therapeutic target in p53-mutant breast cancers

Two ubiquitin ligases, APC/C-Cdh1 and SKP1-CUL1-F (SCF)-beta-TrCP, sequentially regulate glycolysis during the cell cycle

Mapping the binding sites of antibodies utilized in programmed cell death ligand-1 predictive immunohistochemical assays for use with immuno-oncology therapies

SARS-CoV-2 testing and sequencing for international arrivals reveals significant cross border transmission of high risk variants into the United Kingdom

Molecular structure of human geminin

Hexameric ring structure of human MCM10 DNA replication factor

DNA replication licensing in peripheral B-cell lymphoma

DNA replication licensing in somatic and germ cells

Cell cycle markers in clinical oncology

Cdc6 protein causes premature entry into S phase in a mammalian cell-free system

Identification of an arginine-rich motif in human papillomavirus type 1 E1;E4 protein necessary for E4-mediated inhibition of cellular DNA synthesis in vitro and in cells

Standard Reference Material: Cell Line Development and Use of Reference Cell Lines as Standards for External Quality Assurance of Her2 IHC and ISH Testing. Antigen Retrieval Immunohistochemistry Based Research and Diagnostics (Book)

Use of the Monoclonal Antibody DAKO-ERβ (8D5-1) to Measure Oestrogen Receptor Beta in Breast Cancer Cells

Cdc7 kinase is a predictor of survival and a novel therapeutic target in epithelial ovarian carcinoma

Small-molecule mimics of an alpha-helix for efficient transport of proteins into cells

Aberrant expression of minichromosome maintenance proteins 2 and 5, and Ki-67 in dysplastic squamous oesophageal epithelium and Barrett’s mucosa

Phospho-STAT5 and phospho-Akt expression in chronic myeloproliferative neoplasms

Diagnosis of pancreaticobiliary malignancy by detection of minichromosome maintenance protein 5 in bile aspirates

DNA replication licensing and human cell proliferation

Immunoassay for urothelial cancers that detects DNA replication protein Mcm5 in urine

Novel markers of cell kinetics to evaluate progression from cirrhosis to hepatocellular carcinoma

DNA replication licensing factors and aurora kinases are linked to aneuploidy and clinical outcome in epithelial ovarian carcinoma.

Diagnosis of oesophageal cancer by detection of minichromosome maintenance 5 protein in gastric aspirates

Cdc6 protein causes premature entry into S phase in a mammalian cell-free system

Mitogenic growth signalling, DNA replication licensing, and survival are linked in prostate cancer